Introduction

Delirium is an acute neuropsychiatric syndrome characterized by confusion, attentional deficits, and a fluctuating cognitive impairment.1 It is associated with substantial morbidity, functional decline, long-term cognitive deterioration, higher healthcare costs and mortality, contributing up to $152 billion annually in the United States.1–3 Delirium incidence varies widely across healthcare settings, affecting up to 20% of all hospitalized patients and up to 80% of those in intensive care or post-surgical environments.4 Older patients are particularly vulnerable, with the risk of delirium increasing by approximately 2% for every additional year beyond the age of 65.5 As the population ages and longevity increases,6 the number of vulnerable individuals at risk for delirium is expected to rise, emphasizing the need for enhanced understanding of risk factors and effective prevention strategies.

Delirium is a multifactorial condition resulting from a combination of predisposing and precipitating factors including medications.7 Evaluating medications is critical due to their ubiquitous use before, during and after hospitalization and the modifiability of their use. Medications are suggested to be the sole precipitant for 12-39% of cases of delirium.8 Medications with anticholinergic potential, in particular, have been identified as high-risk due to their antagonistic effects on acetylcholine, a neurotransmitter crucial for cognitive functions often disrupted in delirium such as attention, sleep, and memory.9 The widespread prescription of anticholinergic drugs for a plethora of conditions including mental, neurological, respiratory, ophthalmic, and urological disorders,10 coupled with their known negative cognitive impact11–13 underscores the need for a critical evaluation of their use in vulnerable populations.

Existing delirium research has predominantly focused on anticholinergic medication use within hospital settings, neglecting the anticholinergic burden that may carry from regularly prescribed medicines.14 To our knowledge, no review has specifically examined how community-based anticholinergic burden relates to delirium outcomes. By systematically evaluating existing literature in these underexplored settings, we aim to clarify the strength and consistency of this association. The main research question is whether anticholinergic burden of regularly used, community-based medications increases delirium occurrence severity, or duration. Such insights have the potential to refine clinical decision-making, guide prescribing practices, and ultimately inform strategies to prevent or mitigate delirium in vulnerable populations.

Methods

A protocol for this review was prospectively registered with PROSPERO (CRD42023314206). This review is reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Guidelines (PRISMA) .15The review was performed using Covidence®,16 an online tool designed to streamline the systematic review process by facilitating abstract screening, full-text screening, study selection, quality assessment, and data extraction.

Data sources and search Strategy

A comprehensive literature search was conducted across multiple electronic databases including PubMED, EMBASE, PsycINFO, CINAHL, and Web of Science from their inception to November 2023. The search strategy was modified from similar searches created by Egberts et al17 with additional terms added. Terms related to delirium (e.g "delirium", “acute confusion”, “cognitive dysfunction”) were combined with terms related to anticholinergic drugs (e.g “anticholinergic”). The search terms and strategies for all databases are shown in Supplement B. Reference lists of identified articles and relevant reviews were manually searched to ensure comprehensive coverage.

Eligibility Criteria

Studies included if they (i) assessed anticholinergic burden of medicines taken prior to hospital or long-term care facility admission; (ii) reported any of incidence, prevalence, duration of delirium or delirium severity as an outcome; and (iii) included adult participants (≥18 years). Studies were excluded if they (i) focused exclusively on in-hospital anticholinergic exposure; (ii) were case reports, case series, abstracts, study protocols editorials, or review articles; (iii) lacked primary data or clear delirium outcomes, (iv) studies that used a composite measure of sedative and anticholinergic burden, (v) studies that used a consolidated dementia-delirium outcome, (vi) studies with unclear exposure assessment period, (vii) studies not in English.

Study Screening and Selection

All references identified by search queries were added to Covidence®16 and duplicates were removed. One author (H.K) screened titles and abstracts for potentially eligible studies. Two authors (H.K and N.K) independently assessed full-text articles against the eligibility criteria. Disagreements at any stage were resolved through consensus.

Data Extraction

Data was extracted independently by two reviewers using a standardized extraction form. Extracted information included study characteristics (authors, year of publication, country), study design, sample size, participant demographics, method for assessing anticholinergic burden, medication data source, delirium assessment tools, and main findings of each study with regards to the relationship between medications and the outcomes of interest.

Quality assessment

Two reviewers independently assessed the methodological quality of included studies. Cohort and case-control studies were assessed using the Newcastle-Ottawa Scale (NOS).18 The Modified NOS scale was used for cross-sectional studies.19 Each study was evaluated for the adequacy of sample selection, comparability of groups and assessment of outcomes. The score ranged from 0 (lowest degree) to 9 (highest degree). Studies ≤ 4 stars, 5-6 stars, and ≥ 7 stars were defined as low, medium, and high quality respectively. In the event of disagreement on critical appraisal score, agreement was met through discussion between evaluators.

Data Analysis

The data extraction tables were reviewed to determine the most appropriate analysis technique to answer the research question. There was considerable heterogeneity in relation to population characteristics, methods, and data sources to obtain anticholinergic exposure, duration of exposure, follow-up period, outcome measurement and reporting of data. Therefore, a meta-analysis was not feasible. Accordingly, a qualitative narrative review of the literature was performed to assess the association between anticholinergic burden and delirium outcomes.

Results

Study selection

The initial literature search resulted in 4311 with 2437 remaining after duplicate removal. Screening excluded 2324 records, leaving 110 full-text articles for eligibility assessment. Of these, 24 studies met the inclusion criteria and were included (Figure 1).

Figure 1
Figure 1.PRISMA flow diagram of the study selection process

Characteristics of included studies

The 24 studies included were published between 2004 and 2023. They comprised 8 prospective cohorts,20–27 6 retrospective cohorts,28–33 5 cross-sectional studies,34–38 3 case-controls,39–41 1 nested case-control,42 and 1 post-hoc analysis of a randomized control trial.43 Studies were performed in 15 different countries with two being multi-country. In total, 673,938 participants were included (mean age range of 63-86.3, 45.3% male).

Seventeen of the included studies were conducted in hospital settings,20–23,25–27,31–34,36,38–41,43 with 6 specifically examining surgical patients.20–23,32,43 The remaining hospital studies focused on a range of populations including patients admitted to geriatric or medical wards,34,36,38 internal medicine wards,26 and intensive care units (ICU),27 as well as patients admitted for advanced cancer treatment,31 stroke,39 emergency admissions,25,33,41 and rehabilitation.40 Two studies were in a nursing homes24,35 and one at a hospice/home,37 and four were population-based studies using health insurance records28–30,42 (Table 1). One study exclusively included patients with dementia,28 while four excluded patients with dementia.20–22,32

Table 1.Characteristics of included studies
Author, year Study Design Country Population Sample Size Age, in years* Male (n, %) Delirium (n, %)** Delirium Identification Outcomes
Hospital non-surgical
Caeiro, 200439 Case-control Portugal Acute stroke patients in neurology ward 74 63.6 (12.8) 47 (64) 22 DSM-IV, DRS Prevalence
Ceppi, 202340 Case-control Switzerland Rehabilitation patients 625 77.2 (9.9)g, 64.6 (15.7)h 70 (55)g,
225 (45)h
125 Medical records Incidence
Egberts, 201734 Cross-sectional Netherlands Acutely ill patients aged ≥ 65 in geriatric ward 905 81 (7.03) 437 (48.3) 215 (23.8%) DSM-IV, DOSS Prevalence
Lee, 202131 Retrospective Cohort South Korea Stage IV solid cancer patients aged ≥ 65 in medical ward 430 72.6 265 (61.6) 35 (8.1%) Medical records Incidence
Moorey, 201641 Case-control United Kingdom Patients aged ≥ 70 with unplanned medical admission 251 83.97 (6.6) 82 (32.7) 125 (49.8%) CAM, DSM-IV Prevalence
Naja, 201525 Prospective Cohort France Patients aged ≥ 75 admitted for various reasons often on an emergency basis 102 86.3 (5.8) 49 (48) 34.8%-60% CAM Prevalence
Pasina, 201944 Cross-sectional Italy Patients aged ≥ 65 patients in acute geriatric unit 477 85 (6.5)c,
83.4 (6.5)d
64 (42.4)c,
106 (41.8)d
151 (31.7%) 4A’s Test Prevalence
Rigor, 202026 Prospective Cohort Portugal Patients aged ≥ 65 in internal ward 198 79.9 (7.5) 106 (53.5) 56 (28.3%) s-CAM Incidence
Rawle, 202133 Retrospective Cohort United Kingdom Patients aged ≥ 70 and older admitted for ≥ 48 hr for unplanned emergency 577 83.2 (7.4) 237 (41) 175 (30%) s-CAM Prevalence by subtype (full syndromal, subsyndromal)
Vondeling, 202027 Prospective Cohort Netherlands Non-neurological patients admitted to ICU for > 24 hours 1,090 63 (15)c,
58 (16)d
328 (64)c,
328 (57)d
513 (47%) Flowchart based on CAM-ICU Incidence
Wahab, 201938 Cross-sectional Malaysia Patients aged ≥ 60 in geriatric and medical wards 145 71.51 (8.02) 70 (48.3) Not reported Medical records Prevalence
Surgical
Duprey, 202220 Prospective Cohort United States Patients aged ≥70 years undergoing major elective surgery 560 76.6 (5) 52 (39)c,
179 (42)d
134 (23.9%) CAM Incidence
Ferré, 202215 Prospective Cohort France Patients aged ≥ 65 undergoing urgent surgery of hip fracture 67 78 [71-86] 24 (36) 36 (53.7%) CAM Incidence
Heinrich, 202122 Prospective Cohort Germany, Netherlands Patients aged ≥ 65 undergoing elective surgery with expected duration ≥ 60 min 837 74 [70-77]c,
71 [68-75]d
86 (52.1)c,
389 (57.9)d
165 (19.7%) DSM-V, CAM, CAM-ICU, NuDESC Incidence
Herrmann, 202223 Prospective Cohort Germany Patients ≥ 70 undergoing elective surgery with an expected duration ≥ 60 min 899 77.3 (4.9) 455 (50.6) 210 (23.4%) CAM, DSM-V Incidence
Mueller, 202032 Retrospective Cohort Germany Patients aged ≥ 65 scheduled for surgical treatment of gastrointestinal, genitourinary, or gynecological cancers 651 71.8 (4.9) 446 (68.5) 66 (10.1%) CAM-ICU, NuDESC Incidence
Tillemans, 202143 Post-hoc analysis of RCT Netherlands Patients ≥ 70 with intermediate or high risk for postoperative delirium admitted for acute or elective hip surgery 397 82.3e,
82.6f
13 (35.1)e,
8 (25.8) f
68 (15.8%) CAM, DSM-IV Incidence, severity (DRS-R-99), duration
Nursing home or long-term care
Landi, 201424 Prospective Cohort Italy Long-term nursing home residents aged ≥ 65 1,490 83.56 [65.1-106.4] 28.5 (28.5) Not reported NH-CAM Incidence
Oudewortel, 202135 Cross-sectional European Multi-countrym Short- and long-stay nursing home residents 3,924 80a, 84b 1,059 (27) 643 (15.5%) Approximated from recorded symptoms in SHELTER database Prevalence
Pasina, 202137 Cross-sectional Italy Adults admitted and died in hospice or cared for at home by palliative care physicians 461 83 [76.5-88.6]c,
78.1 [71.7-84.9]d
235 (42) 124 (26.9%) 4A’s Test Prevalence
Population based studies
Huang, 201229 Retrospective Cohort Taiwan Patients aged ≥ 65 72,556 73.98 (6.51)i, 74.3 (7.01) j 23,696 (43.17)i, 8,601 (48.68)j 534 (0.73%) ICD-9 codes Incidence
Ah, 201945 Retrospective Cohort South Korea Dementia patients > 60 who started a cholinesterase inhibitor 7,438 39% 60-74 yrs
60.9% ≥ 75 yrs
2,556 (34.4) 298 (4%) ICD codes Incidence
Hwang, 201930 Retrospective Cohort South Korea Persons aged ≥ 65 118,750 75.4 (6.6) 51,748 (43.6) 66 (0.05%) ICD-10 codes Incidence
Hwang, 202142 Nested Case-control South Korea Patients aged ≥ 65 461,034 76.1 (7.1) 180,351 (39.1) 719 ICD-10 codes Incidence

CAM, Confusion Assessment Method; DRS, Delirium Rating Scale; DSM, Diagnostic Statistical Manual of Mental Disorders; ICD, International Classification of Diseases; ICU, Intensive Care Unit; IQR, Interquartile range; Nu-DESC, Nursing Delirium Scale; SD, Standard Deviation; SHELTER, Services and Health for Elderly in Long TERm care; 4AT,
*Age reported as mean (SD or range) or median [Range]
** Percentage not reported for case-control studies
a. Men, b. Women, c. Delirium group, d. non-delirium group, e. Placebo with delirium, f. Haloperidol group, g. cases, h. controls, i. patients taking anticholinergics, j. patients not taking anticholinergics, k. Czech Republic, England, Finland, France, Germany, Italy, and the Netherlands

Details on the methodological quality of the studies included according to the NOS are provided in section B of supplement. Quality scores ranged from 3 to 9 stars (average 7.5 stars). The findings indicate that the evidence-base was largely moderate to high in methodological quality.

Assessment of Delirium

Delirium was using the Diagnostic and Statistical Manual of Mental Disorders criteria (DSM-IV or V, 6 studies),22,23,34,39,41,43 the Confusion Assessment Method in its various forms (CAM, 12 studies),20–27,32,33,41,43 diagnosis codes for delirium according to the international classification of diseases 9th and 10th edition (ICD-9 or 10, 4 studies),28–30,42 4AT test (2 studies),36,37 medical record documentation (3 studies),31,38,40 and the Nu-DESC (1 study).22 One study used recorded pre-specified symptoms indicating acute mental status changes from a cross-sectional study.35 Delirium incidence ranged between 66 of 118750 patients (0.05%)30 to 36 of 67 (53.7%)21 and prevalence ranged between 124 of 461 (26.9%)37 and 125 of 251 (49.8%)41 among included studies (Table 1).

Delirium severity was measured with the Delirium Rating Scale (DRS) in two studies,39,43 however only one43 assessed the possible association between anticholinergic burden and severity. Delirium duration similarly was assessed by only one study.43 In addition, one study further explored the impact of anticholinergic burden on subsyndromal delirium.33

Anticholinergic drug burden measurement

Among the 24 included studies, 22 used anticholinergic scales, with the remainder relying on anticholinergics listed in prior literature21 or pharmacological categories based on national drug formularies.39 The most frequently utilized anticholinergic scales were the Anticholinergic Cognitive Burden scale (ACB, 12 studies),20,22,26,31,33–37,40–42 the Anticholinergic Drug Scale (ADS, 8 studies),20,22,27,32,38,41–43 and the Anticholinergic Risk Scale (ARS, 7 studies).22–24,29,34,35,42 Ah et al. (2019) modified the ACB scale28 and Hwang et al. (2019) modified the ARS scale30 to account for anticholinergics available in their study countries. Less common scales included the list of Chew34 and Coupland’s Anticholinergic Burden Score (ABS).23,46 One study25 combined the ACB and ADS scales to account for the greatest number of possible anticholinergics. Fourteen studies used one scale24,26–33,36–38,40,43 while nine used multiple.20–23,25,34,35,41,42 Studies implemented scales by summing potency points (17 studies), or they were used to identify anticholinergic drugs and subsequently classify patients as users versus non-users (4 studies) or the number of anticholinergics taken by patients (7 studies) (Table 2).

Table 2.Description of anticholinergic exposure and summary findings
Study, year Anticholinergic exposure assessment approach Anticholinergic Scale Used Exposure type and duration Source of exposure ascertainment Include dose or duration in exposure measurement Findings
Hospital non-surgical
Caeiro, 2004 Use versus non-use,
Number of anticholinergic drugs
None Single point – Before admission (unspecified) Medical records None Pre-morbid intake of medications with anticholinergic activity is associated with delirium in stroke patients
Ceppi, 2023 Sum of scale points ACB Single point – At time of admission Interview or questionnaire None High anticholinergic burden was a potential risk factor for incident delirium
Egberts, 2017 Sum of scale points,
Number of anticholinergic drugs
ARS, ACB, List of Chew Single point – At time of admission Medical records or interview None Anticholinergic exposure measured with ARS, but not ACB or list of Chew, was associated with an increased prevalence of delirium on admission and increased post-discharge institutionalization
Lee, 2021 Sum of scale points ACB Single point – At admission Medical records or interview None An increase in ACB during hospitalization was associated with delirium in older patients with cancer
Moorey, 2016 Sum of scale points ACB, ADS Single point – Drugs prescribed within 48 hours of admission Medical records None Anticholinergic burden was not associated with delirium on admission
Naja, 2015 Sum of standardized doses multiplied by anticholinergic burden score Combined ACB-ADS Single point - Not mentioned Medical records or interview Dose Pre-hospital anticholinergic burden was not associated with delirium at any day during hospital admission
Pasina, 2019 Sum of scale points ACB Single point – At admission Interview or questionnaire None There was a dose-response relationship between anticholinergic burden and delirium, but its significance disappeared after adjustment for dementia and nutritional status
Rawle, 2021 Sum of scale points ACB Single point – At admission Not mentioned None Anticholinergic burden was not associated with increased risk of delirium or mortality
Rigor, 2020 Sum of scale points,
Number of anticholinergic drugs used
ACB Single point – At admission Medical records or interview None Pre-hospital anticholinergic burden was associated with delirium but not mortality
Vondeling, 2020 Sum of scale points ADS Single point – At admission Medical records or interview None Exposure to drugs with anticholinergic effects before ICU admission increases the risk of developing delirium during critical illness
Wahab, 2019 Use versus non-use,
Number of anticholinergic drugs used
ADS Single point – At admission Medical records or interview None Use of medicines with anticholinergic properties was associated with falls, confusion, and longer length of stay
Surgical
Duprey, 2022 Sum of scale points,
Number of anticholinergic drugs used
ACB, ADS Single point – 2 weeks before surgery Medical records or visual inspection of medications None No significant association between continuous ACB and ADS and incident delirium
Ferre, 2022 Sum of scale points List from Duran et al.47 and Laroche et al48 for drugs marketed in France Single point – At time of admission Not mentioned None Pre-hospital atropinic burden was not associated with delirium postoperatively
Heinrich,2021 Sum of scale points,
Use versus non-use
ARS, ACB, ADS Single point – Long term medications taken at time of study enrollment Medical records or interview None There was no association between pre-operative anticholinergic load (ARS, ACB, ADS) and delirium
Herrmann, 2022 Sum of scale points,
Number of anticholinergic drugs used
ARS, ABS from Coupland et al .46 Single point – At admission Interview or questionnaire None Preoperative anticholinergic burden was an independent risk factor of postoperative delirium in older adults
Mueller, 2020 Sum of scale points,
Use versus non-use
ADS Single point – At baseline Interview or questionnaire None Higher preoperative anticholinergic burden following long-term medication use was independently associated with the development of postoperative delirium
Tillemans, 2021 Sum of scale points ADS Single point – At admission Not mentioned None Anticholinergic burden was not associated with delirium duration and severity
Nursing home or long-term care
Landi, 2014 Sum of scale points ARS Single point - Medications taken 7 days before baseline Interview or questionnaire None Nursing home residents using anticholinergic medications were at higher risk of delirium
Oudewortel, 2021 Sum of scale points,
Number of anticholinergic drugs used
ARS, ACB Single point – Drugs used in the 3 days before baseline Interview or questionnaire None ACB and ARS scales were associated with delirium in patients with dementia
Pasina, 2021 Sum of scale points ACB Single point – At admission Interview or questionnaire None Anticholinergic burden was associated with increased risk of delirium
Population based
Ah, 2019 Sum of standardized doses multiplied by anticholinergic burden score across duration Modified ACB 3 months Medical records Dose and duration High dose-adjusted anticholinergic burden measured by ACB was an independent prognostic factor for delirium in newly treated dementia patients
Huang, 2012 Use versus non-use ARS Single point during an observation period of 1-year Medical records None Exposure to drugs on ARS scale was associated with higher odds or delirium
Hwang, 2019 Sum of standardized doses multiplied by anticholinergic burden score across duration Modified ARS 92 days Medical records Dose and duration High anticholinergic burden in older adults increases the risk of delirium visits
Hwang, 2021 Sum of standardized doses multiplied by anticholinergic burden score across duration ARS, ACB, ADS, KABS 30 days Medical records Dose and duration High anticholinergic burden as measured by KABS, ARS, ACB, and ADS was associated with emergency visits of delirium

ACB, Anticholinergic cognitive burden scale; ARS, Anticholinergic risk scale; ADS, Anticholinergic drug scale; ABS, Anticholinergic burden scale; KABS, Korean anticholinergic burden scale; ICU, Intensive care unit

Exposure assessment was typically based on a single time point (21 studies), most commonly at hospital admission (12 studies) (Table 2). Others specified distinct time windows for exposure assessment: Huang et al. (2012)29 used an 1-year exposure period, Landi et al. (2014)24 used a 7 day time-frame, Oudewortel et al. (2021)35 considered 3 days, and Moorey et al. (2016)41 48 hours. Duprey et al. (2022)20 considered patients taking anticholinergics if they reported them in an interview 2 weeks before planned hospital admission. Three studies did not specify the time point of anticholinergic assessment22,25,39 and sufficed to indicate medications taken before admission,25,39 or long-term medications taken regularly.22 Among studies using a single time point assessment, only one incorporated doses to quantify anticholinergic burden.25 Longitudinal exposure measures incorporating dosage, duration, and anticholinergic potency was performed by only 3 studies,1–3 all of which calculated an average daily burden score (Equation in Supplement.C). Ah et al. (2019)28 calculated an average daily ACB score over 3 months, Hwang et al. (2019)30 calculated an average ARS score over 3 months, and Hwang et al. (2021)42 calculated average KABS, ARS, ACB, and ADS over 30 days.

Anticholinergics and Delirium Outcomes

Hospitalized non-surgical populations

In non-surgical hospitalized patients, four studies reported a positive association between higher anticholinergic burden measured at or before admission and incident delirium.26,27,31,40 Three of these studies used the ACB scale26,31,40 and one used the ADS27 to identify medicines. The populations included patients in inpatient rehabilitation,40 advanced cancer patients admitted to a medical ward,31 general internal medicine patients,26 and patients admitted to the ICU27 (Table 3).

Table 3.Results of Included Studies
Study, year Anticholinergic scale Drug Exposure; Exposure Period Adjustments Outcome Results
Hospital non-surgical
Ceppi, 2023 ACB Anticholinergics taken by patient at hospital admission, single time point Age, sex, rehabilitation discipline, index date and time span between admission and index date Incident delirium ACB ≥3 vs <3: OR 2.59 (1.41-4.73)*
Lee, 2021 ACB Anticholinergics being taken by patients at hospital admission, single time point Age, Sex, CCI, number of medications, length of stay, types of cancer, admission through emergency department, vital signs, laboratory results Incident delirium Patients with an increase in ACB score vs those with no increase: OR 13.45 (2.78-64.96)*
Rigor, 2020 ACB Anticholinergics being taken by patients at hospital admission – single time point Age, sex, CCI, dementia, number of comorbidities, number of outpatient drugs, number of inpatient drugs, number of outpatient ACB drugs, number of inpatient ACB drugs Incident Delirium ACB:
Number of medications: OR 0.55 (0.27 – 1.13)
Sum of points: OR 1.65 (1.09 – 2.51)*
Vondeling, 2020 ADS Anticholinergics being taken by patients at hospital admission – single time point Age, sex, drinking status, SOFA, APACHE IV, depression, diabetes, hypertension, sepsis or septic shock, cerebrovascular disease, cardiac failure, elective ICU admission (vs emergency), surgery before ICU admission Incident delirium ADS Sum of points:
All patients:
1 vs 0: sHR 1.13 (0.91 - 1.40)
≥2 vs 0: sHR 1.32 (1.06 - 1.64)*
Age ≥ 65 years, no severe sepsis and/or septic shock:
1 vs 0: sHR 1.52 (0.99 - 2.33)*
≥2 vs 0: sHR 2.15 (1.43 - 3.25)*
Age ≥ 65 years, severe sepsis and/or septic shock:
1 vs 0: sHR 0.75 (0.47 - 1.20)
≥2 vs 0: sHR 0.84 (0.51 - 1.39)
Caeiro, 2004 None ACH use vs non-use prior to admission - single time point Non-neuroleptics ACH during hospitalization, medical complications, ACH taken before stroke and ICH Prevalent delirium ACH use vs non-use before stroke: OR 11.3 (1.19-108.2)*
Egberts, 2017 ARS, ACB, List of Chew Anticholinergics taken by patient at hospital admission, single time point Age, sex, number of medications Prevalent delirium ARS:
Use vs non-use: OR 1.73 (1.23 - 2.45)*
Number of medications OR 1.38 (1.10 - 1.73)*
Sum of points:
1-2 vs 0 OR 1.7 (1.16 - 2.49)*
≥ 3 vs 0 OR 1.83 (1.06 - 3.15)*
ACB:
Use vs non-use: OR 1.1 (0.77 - 1.59)
Number of medications: OR 1.07 (0.94 - 1.23)
Sum of points:
1-2 vs 0: OR 0.99 (0.67 - 1.46)
≥3 vs 0: OR 1.39 (0.89 - 2.18)
List of Chew:
Use vs non-use: OR 1.09 (0.78 - 1.51)
number of medications: OR 1.11 (0.94 - 1.31)
Sum of points:
0.5-1 vs 0: OR 1 (0.71 - 1.43)
≥1.5 vs 0: OR 1.34 (0.85 - 2.11)
Moorey, 2016 ACB, ADS Anticholinergics being taken by patients at hospital admission, single time point Age, number of anticholinergics, polypharmacy, anticholinesterase use Prevalent delirium Non-significant, Not reported
Naja, 2015 Combined ACB - ADS Anticholinergics being taken by patients before hospital admission, single time point Age, sex, education, CCI, cognitive level (MMSE), sensorial disturbances, marital status, place of residence (with spouse or other family member, alone or in an institution), socioeconomic level before retirement, perceived health status on a numeric scale (0-10), number of previous hospitalizations, creatinine clearance, serum albumin Prevalent delirium Anticholinergic burden at home with delirium at day 1, day 3, day 5, day 8, day 15 Not significant
Pasina, 2019 ACB Anticholinergics being taken by patients at hospital admission, single time point Age, sex, dementia, cancer, mini nutritional assessment Prevalent delirium ACB sum of points:
1 vs 0: OR 0.93 (0.49-1.79)
2 vs 0: OR 1.01 (0.47-2.16)
3 vs 0: OR 1.81 (0.74-4.47)
4 vs 0: OR 2.19 (0.87-5.53)
≥5 vs 0: OR 2.73 (0.85-8.77)
Wahab, 2019 ADS Anticholinergics being taken by patients at hospital admission, single time point Age, sex, drinking status, smoking status, number of medications, comorbidities Prevalent delirium ADS use vs non-use: OR 3.6 (1.55-8.37)*
Rawle, 2021 ACB Anticholinergics being taken by patients at hospital admission, single time point Univariate Subsyndromal Delirium prevalence,
Full syndromal delirium prevalence
ACB sum of points:
Subsyndromal Delirium
1 vs 0: OR 0.86 (0.49 - 1.51)
2 vs 0: OR 0.88 (0.42 - 1.82)
≥3 vs 0: OR 1.61 (0.89 - 2.94)
Full Syndromal Delirium
1 vs 0: OR 1.06 (0.59 - 1.93)
2 vs 0: OR 0.72 (0.30 - 1.72)
≥3 vs 0: OR 1.49 (0.76 - 2.94)
Surgical
Duprey, 2022 ACB, ADS Anticholinergics taken by patients 2 weeks before surgery, single time point Age, Sex, CCI, Surgery type, Baseline GCP, APACHE-II, The number of medications with anticholinergic activity Incident delirium ACB number of medications: RR: 1.03 (0.88-1.20)
ADS number of medications: RR: 1.03 (0.87-1.22)
Ferre, 2022 Duran et al.114 and Laroche et al115 Anticholinergics being taken by patients at hospital admission, single time point CIRS-G frailty score, prehospital AB, In-hospital AB Incident delirium Non-significant, not reported
Heinrich, 2021 ARS, ACB, ADS Long term medications taken regularly at time of study enrollment, single time point Age, CCI, duration of anesthesia, number of long-term medications Incident delirium ADS:
Number of medications: OR 0.955 (0.62-1.46)
Sum of points: OR 0.928 (0.74 -1.15)
ARS:
Number of medications: OR 0.784 (0.37-1.63)
Sum of points: OR 0.832 (0.56-1.22)
ACB:
Number of medications: OR 1.13 (0.762-1.68)
Sum of points: OR 1.045 (0.84-1.29)
Herrmann, 2022 ARS, ABS67 Long term medications taken for more than 3 days a week at hospital admission, single time point Age, sex, CCI, number of medications, Surgery type, ICU stay, preoperative serum creatinine, type of anesthesia, usage of heart-lung-machine Incident delirium ARS Sum of points: OR 1.54 (1.15-2.02)*
ABS Number of medications: OR 2.74 (1.55-4.49)*
Mueller, 2020 ADS Long term anticholinergics taken every day before hospital admission, single time point Age, Sex, CCI, duration of anesthesia, ASA, severity of surgery, Number of prescribed long-term drugs Incident delirium ADS:
sum of points: OR 1.50 (1.09-2.05)*
use vs non-use: OR 2.21 (1.21-4.04)*
Tillemans, 2021 ADS, Dutch expert panel scale (0-2) Anticholinergics being taken by patients at hospital admission, single time point Univariate Delirium duration and severity Non-significant, Not reported
Nursing home or long term care
Landi, 2014 ARS Anticholinergics received by patients during 7 days preceding baseline assessment, single time point Age, Sex, cognitive level (CPS), depression, schizophrenia, comorbidities, CIRS Incident delirium during 1-year follow up ARS sum of points: OR 1.16 (1.02-1.32)*
Oudewortel, 2021 ARS, ACB Anticholinergics received by patients for 3 days preceding baseline assessment – single time point Age, CCI, cognitive level (CPS) Prevalent delirium Patients with dementia:
ARS sum of points 1.17 (1.04 - 1.31)*
ARS none, moderate, strong 1.26 (0.99 - 1.49)
ACB sum of points 1.14 (1.06 - 1.23)*
ACB none, moderate, strong 1.26 (1.11 - 1.44)*
Patients without dementia:
ARS sum of points 1.07 (0.94 - 1.21)
ARS none, moderate, strong 1.1 (0.86 - 1.43)
ACB sum of points 1.07 (0.97 - 1.18)
ACB none, moderate, strong 1.14 (0.94 - 1.38)
Pasina, 2021 ACB Anticholinergics being taken by patients at hospital admission – single time point Age, sex, dementia, cancer, karnofsky performance score, days of palliative assistance, care setting (hospice or home). Prevalent delirium ACB sum of points:
1 vs 0: OR 0.62 (0.16-2.43)
2 vs 0: OR 0.44 (0.11-1.72)
3 vs 0: OR 1.85 (0.52-6.61)
4 vs 0: OR 1.53 (0.39-6.00)
≥5 vs 0: OR 2.25 (0.69-7.28)
Population based
Huang, 2012 ARS Patients taking at least 1 anticholinergic during a 1-year observation period Age, sex, CCI, number of medications, presence of chronic disease Incident delirium during 6 month follow up ARS use vs non-use: OR 1.51 (1.18-1.93)*
Ah, 2019 Modified ACB Average daily ACB, 3 months Age, sex, depression, diabetes, hyperlipidemia, stroke, hypertension, sedative drugs, parkinson's disease, use of prescription drugs with gingko extract during the first 90 days Incident delirium during 1 year follow up Average daily ACB score >3: HR 1.52 (1.17-1.96)*
Hwang, 2019 Modified ARS Average daily ARS, 3 months Age, sex, insurance type, comorbid conditions, sedative drugs, polypharmacy, excessive polypharmacy, exposure to sedative drugs, warfarin, insulin, digoxin Incident delirium during 3 month follow up Average daily ARS ≥2 vs ≤2: HR 2.05 (1.13 - 3.73)*
Hwang, 2021 ARS, ACB, ADS, KABS Average daily anticholinergic burden, 30 days Age, Sex, CCI, insurance type, comorbid conditions, sedative drugs, concomitant chronic medications prescribed for more than 18 days during a month, use of digoxin, use of Insulin, use of Warfarin Incident delirium during 6 month follow up Average daily KABS
1 vs 0: 1.36 (1.12-1.66)*
2 vs 0: 1.91 (1.46-2.50)*
≥3 vs 0: 2.96 (2.28-3.83)*
Average daily ARS
1 vs 0: 1.14 (0.92-1.41)
2 vs 0: 1.92 (1.32-2.77)*
≥3 vs 0: 2.32 (1.33-4.03)*
Average Daily ACB
1 vs 0: 1.05 (0.86-1.26)
2 vs 0: 1.57 (1.21-2.04) *
≥3 vs 0: 2.22 (1.69-2.90)*
Average daily ADS
1 vs 0 1.39 (1.15-1.68)*
2 vs 0:1.56 (1.19-2.05)*
≥3 vs 0: 2.55 (1.91-3.40)*

ACB, Anticholinergic cognitive burden scale; ARS, Anticholinergic risk scale; ADS, Anticholinergic drug scale; ABS, Anticholinergic burden scale; KABS, Korean anticholinergic burden scale; ICU, Intensive care unit; ACH, Anticholinergics; MMSE, Mini mental state examination; CCI, Charlson comorbidity index; ASA, American Society of Anesthesiologists physical status classification; CPS, Cognitive performance scale; SOFA, Sequential organ failure assessment; APACHE, Acute physiology and chronic health evaluation score; CIRS, Cumulative illness rating scale; GCP, General cognitive performance score ;OR, Odds ratio; RR, Relative risk; sHR, Hazard ratio
*Represents significant associations determined as p ≤0.05

Prevalent delirium was positively associated with anticholinergic burden measured at a single time point on or before hospitalization in three34,38,39 of seven25,33,34,36,38,39,41 studies, examining acutely ill patients admitted to geriatric wards,34,38 and stroke patients.39 These three studies utilized different methods to quantify exposure: one used the ARS scale,34 another categorized patient based on ADS-defined users versus non-users, and the third employed pharmacologic categories rather than standardized scales (Table 3).

Surgical populations

In surgical populations, two23,32 of five20–23,32 studies demonstrated anticholinergic burden measured at or before admission was positively associated with incident delirium. Herrmann et al. (2022)23 and Mueller et al. (2020)32 both reported a similar elevated adjusted-risk per anticholinergic scale point (54% and 49% respectively), although they were used different scales (ARS and ADS respectively). The remaining three surgical studies did not detect a similar association20–22 (Table 3).

Nursing Home and Long-term care residents

In nursing home settings, Landi et al (2014)24 reported a 16% higher risk of incident delirium for each additional ARS point measured at a single timepoint. Similarly Oudewortel et al. (2021),35 observed a 17% higher risk of prevalent delirium for each additional ARS point and a 14% higher risk for each ACB point, though this association was restricted to patients with dementia. In hospice or home care settings, one study identified a relationship between anticholinergic burden (ACB scale) and prevalent delirium37 (Table 3).

Population-level studies

There were four population-based studies incorporating national health insurance claims that consistently supported an association between anticholinergic burden and delirium risk.28–30,42(p2) Of these, three studies used an average daily anticholinergic score to characterize burden. Longer exposure durations and measures that accounted for dose and potency typically yielded stronger associations. Hwang et al. (2021)42 reported increased odds of delirium with higher categorized ACB, ARS, ADS, and KABS scores with dose-response relationships evident across scales. Similarly, Ah et al. (2019)28 found that an average daily ACB score >3 increased the hazard of developing delirium (HR 1.52) (Table 3).

Delirium Severity and Duration

Only one study43 specifically examined the relationship between anticholinergic drug burden and delirium severity or duration in surgical patients population, reporting no significant results.

Discussion

This systematic review examined the relationship between pre-admission anticholinergic drug burden and delirium outcomes. Across 24 included studies, 11 identified a positive association between higher anticholinergic burden and increased risk of incident delirium, while 4 reported a similar association with prevalent delirium. However, substantial variability in measurement methods, populations, and study designs complicates drawing definitive conclusions.

A central challenge identified in this review was the absence of a standardized method to quantify anticholinergic exposure. Among the included studies, there were 7 unique scales employed (ACB, ADS, ARS, List of Chew, ABS, Duran and La roche, KABS), and 3 modified scales (mACB, mARS, ACB-ADS). These tools differ in their derivation, including medications, and potency scoring ratings. Nine studies20–23,25,34,35,41,42 used multiple scales reflecting the lack of a universally optimal tool. Measurement approaches varied even within the same scale: some studies assessed the number of anticholinergic medications20 or categorized patients as users versus non-users,29,38,39. Others summed potency scores, and either considered anticholinergic burden a continuous variable22–24,32 or categorical thresholds.27,33,36,37,40,42,44 Such methodological heterogeneity complicates cross-study comparisons and reduces the clinical utility of findings.

The most utilized tools were the ACB, ARS, and ADS scales, in line with prior research by Salahudeen et al. (2015).13 Among these, the ARS scale showed the most consistent association with delirium, showing a positive relationship in 7 of 8 studies in line with a prior review.17 In contrast, the ACB and ADS scales were less consistent, showing associations in 5 of 12 and 4 of 8 studies, respectively. The ARS’s inclusion of both central and peripheral anticholinergic effects may explain its higher predictive value. Higher anticholinergic burden scores (e.g., ARS≥3) were consistently associated with delirium whereas lower scores had inconsistent effects.

Most of the studies in this review identified anticholinergic use at a single time point, commonly at hospital admission. Three studies29,42,45 however, measured anticholinergic use over an extended period, calculating an average ACB or ARS score by incorporating prescribed dosages over predefined durations. These studies consistently found a significant positive association between prolonged anticholinergic use and delirium risk. Specifically, two studies30,45 examined 3-month exposure periods, and one42 used a 30-day period. This suggests that long-term anticholinergic burden may offer greater predictive value for delirium risk. Notably, no study has used cumulative total standardized doses to assess delirium, a method that is not solely dependent on scale scores44,49 and could provide a more nuanced understanding of dose-response effects independent of specific scale scores. Research in related fields, such as dementia, has demonstrated that cumulative medication exposure over several years can significantly increase dementia risk.46,49 Further studies are needed to see if a similar relationship exists for delirium.

A notable gap in the current literature is the limited focus on core outcomes50 of delirium beyond incidence or prevalence. Only one study in this review examined the relationship between anticholinergic burden and delirium severity and it found no significant association43 between the ADS and delirium severity as measured by the (DRS-R-98). In an earlier study by Han et al. (2001)51 examining in-hospital anticholinergic burden among older inpatients, anticholinergic exposure was significantly associated with delirium severity, as measured with the Delirium Index. Given that delirium severity and duration may affect mortality,52 cognitive and functional decline,53 and being discharged home,54 future research should incorporate standardized severity measures, and evaluate whether anticholinergics influence the depth or persistence of delirium symptoms.

There are several limitations to this study. First, the studies included in this review used retrospective cohort designs or cross-sectional analyses, which limit the ability to infer causality. Although many of the studies controlled for potential confounders, such as age, comorbidities, and polypharmacy, there remains the possibility of residual confounding. For example, patients taking anticholinergic medications may have underlying conditions (e.g., dementia, urinary incontinence) that independently increase their risk for delirium. While the studies tried to adjust for such factors, the observational nature of most studies precludes definitive conclusions about the causative role of anticholinergic burden in the development of delirium. Finally, the potential for publication bias also exists, as studies with null findings may be less likely to be published and included in the review. Other than the variability in measurement of anticholinergic burden, heterogeneity in the study designs, populations, and methodologies poses a significant challenge for drawing definitive conclusions. Studies varied in terms of the populations studied (e.g., general hospital populations, patients undergoing surgery, cancer patients, and dementia patients) and settings (hospital, nursing home, hospice). Furthermore, differences in the tools, employed to assess delirium (e.g., CAM, DSM criteria, ICD codes) may contribute to the inconsistent findings across studies. Lastly, delirium itself is a complex syndrome with the potential to include multiple neurobiological mechanisms.9 While a disruption of cholinergic function can be sufficient to trigger delirium in some, this work does not clarify whether anticholinergic medications are sufficient to result in delirium or serve to increase vulnerability for a different mechanism to result in delirium.

This review has several strengths, including a comprehensive search strategy across multiple databases and the inclusion of a wide range of study designs and populations. By focusing specifically on outpatient and nursing home anticholinergic exposure, this review addresses a gap in the literature, as most prior studies have concentrated on anticholinergic use during hospital stays or have not clearly described time of anticholinergic exposure measurement. For example, Vondeling et al. (2020)27 in ICU patients reported a significantly higher risk of delirium in patients with outpatient prehospital ADS scores ≥2, whereas in a previous study of a similar population Wolters et al. (2015)55 the ADS scale of inpatient medicines was not associated with delirium at any level.

Conclusion

In conclusion, while this review supports an association between elevated anticholinergic burden and delirium, variability in measurement methods, limited longitudinal data, and a focus predominantly on incidence and prevalence rather than severity or duration restricts the generalizability and clinical applicability of the findings. Addressing these gaps through standardized methodologies, comprehensive exposure assessments, and prospective studies will be critical to understanding the true impact of anticholinergic burden on delirium and informing evidence-based interventions.